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Transport in Caco-2 cell monolayers of antidiabetic cucurbitane triterpenoids from Momordica charantia fruits.
- Source :
-
Planta medica [Planta Med] 2014 Jul; Vol. 80 (11), pp. 907-11. Date of Electronic Publication: 2014 Aug 12. - Publication Year :
- 2014
-
Abstract
- Bitter melon, the fruit of Momordica charantia L. (Cucurbitaceae), is a widely-used treatment for diabetes in traditional medicine systems throughout the world. Various compounds have been shown to be responsible for this reputed activity, and, in particular, cucurbitane triterpenoids are thought to play a significant role. The objective of this study was to investigate the gastrointestinal transport of a triterpenoid-enriched n-butanol extract of M. charantia using a two-compartment transwell human intestinal epithelial cell Caco-2 monolayer system, simulating the intestinal barrier. Eleven triterpenoids in this extract were transported from the apical to basolateral direction across Caco-2 cell monolayers, and were identified or tentatively identified by HPLC-TOF-MS. Cucurbitane triterpenoids permeated to the basolateral side with apparent permeability coefficient (P app) values for 3-β-7-β,25-trihydroxycucurbita-5,23(E)-dien-19-al and momordicines I and II at 9.02 × 10(-6), 8.12 × 10(-6), and 1.68 × 10(-6)cm/s, respectively. Also, small amounts of these triterpenoids were absorbed inside the Caco-2 cells. This is the first report of the transport of the reputed antidiabetic cucurbitane triterpenoids in human intestinal epithelial cell monolayers. Our findings, therefore, further support the hypothesis that cucurbitane triterpenoids from bitter melon may explain, at least in part, the antidiabetic activity of this plant in vivo.<br /> (Georg Thieme Verlag KG Stuttgart · New York.)
- Subjects :
- Biological Transport
Caco-2 Cells
Chromatography, High Pressure Liquid
Fruit chemistry
Glycosides chemistry
Glycosides isolation & purification
Humans
Hypoglycemic Agents chemistry
Hypoglycemic Agents isolation & purification
Mass Spectrometry
Plants, Medicinal
Sterols chemistry
Sterols isolation & purification
Sterols metabolism
Triterpenes chemistry
Triterpenes isolation & purification
Diabetes Mellitus drug therapy
Glycosides metabolism
Hypoglycemic Agents metabolism
Momordica charantia chemistry
Triterpenes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0221
- Volume :
- 80
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Planta medica
- Publication Type :
- Academic Journal
- Accession number :
- 25116119
- Full Text :
- https://doi.org/10.1055/s-0034-1382837