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Genetic characterization of porcine kobuvirus and detection of coinfecting pathogens in diarrheic pigs in Jiangsu Province, China.
- Source :
-
Archives of virology [Arch Virol] 2014 Dec; Vol. 159 (12), pp. 3407-12. Date of Electronic Publication: 2014 Aug 14. - Publication Year :
- 2014
-
Abstract
- In this study, 396 samples from diarrheic pigs on 46 pig farms in Jiangsu Province, China, were analyzed by RT-PCR. One-hundred eighty-one pigs from 37 farms tested positive for porcine kobuvirus (PKV). Phylogenetic analysis of the 3D gene from 19 isolates showed sequence homology of 88.0 %-100 % and 69.4 %-100 % for nucleotides and amino acids, respectively, while similarity to isolates of other kobuviruses was 69.6 %-78.8 % and 27.8 %-56.9 %, respectively. One-hundred eighty-five samples contained two or more pathogens, and 31/68 PKV-positive samples tested positive for other diarrheic pathogens, confirming the existence of PKV infection and coinfection.
- Subjects :
- Animals
China
Cluster Analysis
Diarrhea virology
Kobuvirus classification
Phylogeny
Picornaviridae Infections virology
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Swine
Viral Proteins genetics
Diarrhea veterinary
Kobuvirus genetics
Kobuvirus isolation & purification
Picornaviridae Infections veterinary
Swine Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 159
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 25119679
- Full Text :
- https://doi.org/10.1007/s00705-014-2204-2