Back to Search
Start Over
Isolation of arginine kinase from Apis cerana cerana and its possible involvement in response to adverse stress.
- Source :
-
Cell stress & chaperones [Cell Stress Chaperones] 2015 Jan; Vol. 20 (1), pp. 169-83. Date of Electronic Publication: 2014 Aug 19. - Publication Year :
- 2015
-
Abstract
- Arginine kinases (AK) in invertebrates play the same role as creatine kinases in vertebrates. Both proteins are important for energy metabolism, and previous studies on AK focused on this attribute. In this study, the arginine kinase gene was isolated from Apis cerana cerana and was named AccAK. A 5'-flanking region was also cloned and shown to contain abundant putative binding sites for transcription factors related to development and response to adverse stress. We imitated several abiotic and biotic stresses suffered by A. cerana cerana during their life, including heavy metals, pesticides, herbicides, heat, cold, oxidants, antioxidants, ecdysone, and Ascosphaera apis and then studied the expression patterns of AccAK after these treatments. AccAK was upregulated under all conditions, and, in some conditions, this response was very pronounced. Western blot and AccAK enzyme activity assays confirmed the results. In addition, a disc diffusion assay showed that overexpression of AccAK reduced the resistance of Escherichia coli cells to multiple adverse stresses. Taken together, our results indicated that AccAK may be involved of great significance in response to adverse abiotic and biotic stresses.
- Subjects :
- 5' Flanking Region genetics
Amino Acid Sequence
Animals
Arginine Kinase genetics
Arginine Kinase isolation & purification
Base Sequence
Bees genetics
Binding Sites
Escherichia coli metabolism
Herbicides toxicity
Metals, Heavy toxicity
Molecular Sequence Data
Organothiophosphorus Compounds toxicity
Pesticides toxicity
Phylogeny
Sequence Alignment
Temperature
Transcription Factors chemistry
Transcription Factors metabolism
Transcriptome drug effects
Up-Regulation drug effects
Arginine Kinase metabolism
Bees enzymology
Stress, Physiological
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1268
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell stress & chaperones
- Publication Type :
- Academic Journal
- Accession number :
- 25135575
- Full Text :
- https://doi.org/10.1007/s12192-014-0535-2