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Time-dependent degenerative transformations in the lipidome of chalazia.
- Source :
-
Experimental eye research [Exp Eye Res] 2014 Oct; Vol. 127, pp. 261-9. Date of Electronic Publication: 2014 Aug 19. - Publication Year :
- 2014
-
Abstract
- The aim of this prospective study was to conduct histopathologic and lipidomic analyses of chalazia, in order to evaluate time-dependent changes in the lesion. Samples of surgically excised chalazia were collected over a period of 12 months from 10 patients (mean age 41 years; range, 23-58) with clinically diagnosed chalazia, who underwent scheduled surgery. The ages of chalazia varied from 2 to 28 weeks. To confirm the clinical diagnoses, the morphology of collected tissue samples was evaluated histologically after hematoxylin and eosin staining. The lipids from individual chalazia were analyzed by high-performance liquid chromatography-mass spectrometry and compared with authentic lipid standards and with the lipids of meibum collected from normal controls. We observed gradual, lesion age-dependent transformation of the lipidome of chalazia from an almost normal meibum-like composition to a very different kind of lipidome. A rapid initial increase in the free cholesterol content was followed by a gradual replacement of extremely long chain meibomian-type lipids with a mixture of shorter-chain cholesteryl esters of the C14-C18 family, triacylglycerols, ceramides, phospholipids and sphingomyelins. In addition, a rapid disappearance of wax esters and cholesteryl esters of (1-O)-acyl-omega-hydroxy fatty acids from the lipidome of aging chalazia was observed. Our results are indicative of dramatic, time-dependent changes in the lesion that may involve cholesterol as a trigger and/or a marker of subsequent degeneration of the meibomian lipidome. We hypothesize that early inhibition of these transformations may be useful in reversing the course of the disease.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Ceramides metabolism
Chalazion pathology
Chalazion surgery
Cholesterol metabolism
Chromatography, High Pressure Liquid
Female
Humans
Lipid Metabolism
Male
Mass Spectrometry
Meibomian Glands metabolism
Middle Aged
Phospholipids metabolism
Prospective Studies
Time Factors
Triglycerides metabolism
Young Adult
Chalazion metabolism
Lipids analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0007
- Volume :
- 127
- Database :
- MEDLINE
- Journal :
- Experimental eye research
- Publication Type :
- Academic Journal
- Accession number :
- 25150086
- Full Text :
- https://doi.org/10.1016/j.exer.2014.08.008