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Metabolic profiling of Gynostemma pentaphyllum extract in rat serum, urine and faeces after oral administration.
- Source :
-
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2014 Oct 15; Vol. 969, pp. 42-52. Date of Electronic Publication: 2014 Aug 12. - Publication Year :
- 2014
-
Abstract
- Folk drug Gynostemma pentaphyllum (Thunb.) Makino contains many biologically active phytochemicals which have been demonstrated to be effective against chronic diseases. As in vivo anti-tumor experiments of G. pentaphyllum extract (GP) show much stronger antitumor activities than in vitro, it is important and necessary to understand the metabolic study of GP. A sensitive and specific U-HPLC-MS method was utilized for the first time to rapidly identify gypenosides and its possible metabolites in rat serum, urine, and faeces after oral administration. Solid phase extraction was utilized in the sample preparation. Negative Electrospray ionisation (ESI) mass spectrometry was used to discern gypenosides and its possible metabolites in rat samples. As a result, after oral administration, a total of seven metabolites of G. pentaphyllum extract were assigned, two from the rat serum and seven both from the rat urine and faeces. As metabolites of G. pentaphyllum extract, all of them have never been reported before.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Administration, Oral
Animals
Chromatography, High Pressure Liquid methods
Drugs, Chinese Herbal administration & dosage
Drugs, Chinese Herbal metabolism
Feces chemistry
Gynostemma metabolism
Limit of Detection
Linear Models
Male
Mass Spectrometry
Plant Extracts administration & dosage
Plant Extracts analysis
Plant Extracts chemistry
Plant Extracts metabolism
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Gynostemma chemistry
Metabolomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-376X
- Volume :
- 969
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 25151329
- Full Text :
- https://doi.org/10.1016/j.jchromb.2014.08.003