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Endothelial deficiency of L1 reduces tumor angiogenesis and promotes vessel normalization.
- Source :
-
The Journal of clinical investigation [J Clin Invest] 2014 Oct; Vol. 124 (10), pp. 4335-50. Date of Electronic Publication: 2014 Aug 26. - Publication Year :
- 2014
-
Abstract
- While tumor blood vessels share many characteristics with normal vasculature, they also exhibit morphological and functional aberrancies. For example, the neural adhesion molecule L1, which mediates neurite outgrowth, fasciculation, and pathfinding, is expressed on tumor vasculature. Here, using an orthotopic mouse model of pancreatic carcinoma, we evaluated L1 functionality in cancer vessels. Tumor-bearing mice specifically lacking L1 in endothelial cells or treated with anti-L1 antibodies exhibited decreased angiogenesis and improved vascular stabilization, leading to reduced tumor growth and metastasis. In line with these dramatic effects of L1 on tumor vasculature, the ectopic expression of L1 in cultured endothelial cells (ECs) promoted phenotypical and functional alterations, including proliferation, migration, tubulogenesis, enhanced vascular permeability, and endothelial-to-mesenchymal transition. L1 induced global changes in the EC transcriptome, altering several regulatory networks that underlie endothelial pathophysiology, including JAK/STAT-mediated pathways. In particular, L1 induced IL-6-mediated STAT3 phosphorylation, and inhibition of the IL-6/JAK/STAT signaling axis prevented L1-induced EC proliferation and migration. Evaluation of patient samples revealed that, compared with that in noncancerous tissue, L1 expression is specifically enhanced in blood vessels of human pancreatic carcinomas and in vessels of other tumor types. Together, these data indicate that endothelial L1 orchestrates multiple cancer vessel functions and represents a potential target for tumor vascular-specific therapies.
- Subjects :
- Animals
Blood Vessels
Capillary Permeability
Carcinoma metabolism
Cell Movement
Cell Proliferation
Female
Hemangioma metabolism
Humans
Interleukin-6 metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Nude
Mice, Transgenic
Neoplasm Metastasis
Neural Cell Adhesion Molecule L1 genetics
Pancreatic Neoplasms metabolism
Permeability
Phenotype
RNA Interference
Receptor, TIE-2 genetics
STAT3 Transcription Factor metabolism
Endothelial Cells cytology
Endothelium, Vascular pathology
Neoplasms blood supply
Neovascularization, Pathologic
Neural Cell Adhesion Molecule L1 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1558-8238
- Volume :
- 124
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The Journal of clinical investigation
- Publication Type :
- Academic Journal
- Accession number :
- 25157817
- Full Text :
- https://doi.org/10.1172/JCI70683