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TNF-α promoter single nucleotide polymorphisms and haplotypes associate with susceptibility of immune thrombocytopenia in Chinese adults.
- Source :
-
Human immunology [Hum Immunol] 2014 Sep; Vol. 75 (9), pp. 980-5. Date of Electronic Publication: 2014 Aug 23. - Publication Year :
- 2014
-
Abstract
- Objective: Tumor necrosis factor-alpha (TNF-α) participates as a candidate susceptibility factor for immune thrombocytopenia (ITP). This study attempted to investigate the association between five single nucleotide polymorphisms (SNPs) spanning the TNF-α promoter and the susceptibility of primary ITP in Chinese Han adults.<br />Methods: In 215 adult primary ITP patients and 206 healthy controls, SNPs were detected by PCR-RFLP and PCR-SSP. The χ(2) test or fisher's exact test was used to compare frequencies of genotypes and alleles between patients and controls. Haplotypes were analyzed with the SHEsis online program. TNF-α, IFN-γ and Galectin-9 mRNA of 35 newly diagnosed adult ITP patients and 35 healthy controls were detected by qRT-PCR.<br />Results: The haplotype GGC (-238G/-308G/-857C) of TNF-α promoter was significantly associated with a decreased susceptibility of primary ITP, especially in males. The relative levels of mRNA expression of TNF-α, IFN-γ and Gal-9 in adult active primary ITP patients was significantly up-regulated compared with patients in remission and controls.<br />Conclusions: This study represented the first report that the haplotype GGC of TNF-α was differentially associated with the susceptibility of primary ITP in Chinese Han adults. The up-regulation of TNF-α, IFN-γ and Galectin-9 was significantly correlated with active primary ITP in adult patients.<br /> (Copyright © 2014 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
Asian People genetics
China
DNA Primers
Female
Galectins
Gene Expression
Gene Frequency
Genetic Predisposition to Disease ethnology
Genotype
Humans
Interferon-gamma genetics
Linkage Disequilibrium
Male
Middle Aged
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Purpura, Thrombocytopenic, Idiopathic ethnology
Reverse Transcriptase Polymerase Chain Reaction
Sex Factors
Young Adult
Genetic Predisposition to Disease genetics
Haplotypes
Polymorphism, Single Nucleotide
Promoter Regions, Genetic genetics
Purpura, Thrombocytopenic, Idiopathic genetics
Tumor Necrosis Factor-alpha genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1166
- Volume :
- 75
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Human immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25158149
- Full Text :
- https://doi.org/10.1016/j.humimm.2014.08.197