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ZAT11, a zinc finger transcription factor, is a negative regulator of nickel ion tolerance in Arabidopsis.

Authors :
Liu XM
An J
Han HJ
Kim SH
Lim CO
Yun DJ
Chung WS
Source :
Plant cell reports [Plant Cell Rep] 2014 Dec; Vol. 33 (12), pp. 2015-21. Date of Electronic Publication: 2014 Aug 28.
Publication Year :
2014

Abstract

Key Message: ZAT11, a Zinc Finger of Arabidopsis Thaliana 11, is a dual-function transcriptional regulator that positively regulates primary root growth but negatively regulates Ni (2+) tolerance. Zinc Finger of Arabidopsis Thaliana 11 (ZAT11) is a C2H2-type zinc finger protein that has been reported to function as an active transcriptional repressor. However, the biological function of ZAT11 remains unknown. Here we show that GFP-tagged ZAT11 is targeted to the nucleus. Analysis of plants expressing ZAT11 promoter-GUS showed that ZAT11 is highly expressed in roots and particularly in root tips. To identify the biological function of ZAT11, we constructed three independent lines of ZAT11 overexpressing transgenic plant (ZAT11 OE). ZAT11 OE enhanced the elongation of primary root but reduced the metal tolerance against nickel ion (Ni(2+)). The reduced Ni(2+) tolerance of ZAT11 OE was correlated with decreased accumulation of Ni(2+) in plants. The decreased accumulation of Ni(2+) in ZAT11 OE was caused by the reduced transcription of a vacuolar Ni(2+) transporter gene. Taken together, our results suggest that ZAT11 is a dual function transcriptional regulator that positively regulates primary root growth but negatively regulates Ni(2+) tolerance.

Details

Language :
English
ISSN :
1432-203X
Volume :
33
Issue :
12
Database :
MEDLINE
Journal :
Plant cell reports
Publication Type :
Academic Journal
Accession number :
25163803
Full Text :
https://doi.org/10.1007/s00299-014-1675-7