Back to Search
Start Over
Fenofibrate increases radiosensitivity in head and neck squamous cell carcinoma via inducing G2/M arrest and apoptosis.
- Source :
-
Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2014; Vol. 15 (16), pp. 6649-55. - Publication Year :
- 2014
-
Abstract
- Radiation therapy is an important treatment for head and neck squamous cell carcinoma (HNSCC). However, how to promote radiation sensitivity in HNSCC remains a challenge. This study aimed to investigate the radiosensitizing effects of fenofibrate on HNSCC and explore the underlying mechanisms. HNSCC cell lines CNE-2 and KB were subjected to ionizing radiation (IR), in the presence or absence of fenofibrate treatment. Cell growth and survival, apoptosis and cell cycle were evaluated. In addition, CNE-2 cells were xenografted into nude mice and subjected to IR and/ or fenofibrate treatment. The expression of cyclinB and CDK1 was detected by Western blotting. Our results showed that fenofibrate efficiently radiosensitized HNSCC cells and xenografts in mice, and induced apoptosis and G2/M arrest via reducing the activity of the CDK1/cyclinB1 kinase complex. These data suggest that fenofibrate could be a promising radiosensitizer for HNSCC radiotherapy.
- Subjects :
- Animals
Apoptosis drug effects
CDC2 Protein Kinase
Caspase 3 biosynthesis
Cell Line, Tumor
Cell Proliferation drug effects
Cell Proliferation radiation effects
Cell Survival drug effects
Cell Survival radiation effects
Cyclin B1 antagonists & inhibitors
Cyclin B1 metabolism
Cyclin-Dependent Kinases antagonists & inhibitors
Cyclin-Dependent Kinases metabolism
Humans
Male
Mice
Mice, Inbred BALB C
Radiation, Ionizing
Squamous Cell Carcinoma of Head and Neck
Xenograft Model Antitumor Assays
Apoptosis radiation effects
Carcinoma, Squamous Cell radiotherapy
Fenofibrate pharmacology
Head and Neck Neoplasms radiotherapy
M Phase Cell Cycle Checkpoints drug effects
Radiation-Sensitizing Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2476-762X
- Volume :
- 15
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Asian Pacific journal of cancer prevention : APJCP
- Publication Type :
- Academic Journal
- Accession number :
- 25169503
- Full Text :
- https://doi.org/10.7314/apjcp.2014.15.16.6649