Red-shifted fluorescent aminated derivatives of a conformationally locked GFP chromophore.

Authors :: Baranov MS
Solntsev KM
Baleeva NS
Mishin AS
Lukyanov SA
Lukyanov KA
Yampolsky IV
Source :: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2014 Oct 06; Vol. 20 (41), pp. 13234-41. Date of Electronic Publication: 2014 Aug 29.
Publication Year :: 2014
Abstract: A novel class of fluorescent dyes based on conformationally locked GFP chromophore is reported. These dyes are characterized by red-shifted spectra, high fluorescence quantum yields and pH-independence in physiological pH range. The intra- and intermolecular mechanisms of radiationless deactivation of ABDI-BF2 fluorophore by selective structural locking of various conformational degrees of freedom were studied. A unique combination of solvatochromic and lipophilic properties together with "infinite" photostability (due to a dynamic exchange between free and bound dye) makes some of the novel dyes promising bioinspired tools for labeling cellular membranes, lipid drops and other organelles.<br /> (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Amination
Fluorescent Dyes metabolism
Green Fluorescent Proteins chemistry
Green Fluorescent Proteins genetics
HeLa Cells
Humans
Hydrogen-Ion Concentration
Microscopy, Confocal
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Solvents chemistry
Spectrometry, Fluorescence
Fluorescent Dyes chemistry
Green Fluorescent Proteins metabolism

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