Immobilization of trypsin on plasma prepared Ag/PPAni nanocomposite film for efficient digestion of protein.

Authors :: Gogoi D
Barman T
Choudhury B
Khan M
Chaudhari Y
Dehingia M
Pal AR
Bailung H
Chutia J
Source :: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2014 Oct; Vol. 43, pp. 237-42. Date of Electronic Publication: 2014 Jul 11.
Publication Year :: 2014
Abstract: This work demonstrates the efficacy of a support matrix prepared by plasma process for trypsin immobilization without any surface activator. Plasma polymerization cum sputtering process is used to prepare the nanocomposite support matrix. Plasma sputtered silver nanoparticles (AgNPs) are uniformly embedded into plasma polymerized aniline (PPAni) film. Various characterization tools are employed to study the surface morphology, microstructure and chemical composition of the support matrices. Trypsin is immobilized onto the support matrix via the formation of covalent bond between them. Plasma generated free radicals on composite films activate the support matrix and make it efficient for increasing the tertiary enzyme stability via multipoint covalent attachment. Trypsin immobilized onto Ag/PPAni matrix has more hydrolyzing capacity of bovine serum albumin (BSA) than free trypsin as well as trypsin immobilized onto PPAni films.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)

Subjects :: Electrophoresis, Polyacrylamide Gel
Hydrolysis
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Spectroscopy, Fourier Transform Infrared
Enzymes, Immobilized metabolism
Metal Nanoparticles
Nanocomposites
Proteins metabolism
Silver chemistry
Trypsin metabolism

Language :: English
ISSN :: 1873-0191
Volume :: 43
Database :: MEDLINE
Journal :: Materials science & engineering. C, Materials for biological applications
Publication Type :: Academic Journal
Accession number :: 25175210
Full Text :: https://doi.org/10.1016/j.msec.2014.07.025

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