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Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2014 Aug 29; Vol. 19 (9), pp. 13432-47. Date of Electronic Publication: 2014 Aug 29. - Publication Year :
- 2014
-
Abstract
- Momordica charantia Linn. is used as an edible and medicinal vegetable in sub-tropical areas. Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides. In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2. The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo. The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice. Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses. The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively. Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum. MCP1 is mainly composed of glucose and galactose, and MCP2 is mainly composed of glucose, mannose and galactose. The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
- Subjects :
- Animals
Cell Line, Tumor
Cell Proliferation
Cytotoxicity, Immunologic drug effects
Fruit chemistry
Hemolysin Proteins biosynthesis
Immunologic Factors isolation & purification
Inhibitory Concentration 50
Killer Cells, Natural drug effects
Killer Cells, Natural metabolism
Macrophages drug effects
Macrophages metabolism
Male
Mice
Phagocytosis drug effects
Plant Extracts isolation & purification
Polysaccharides isolation & purification
Spectroscopy, Fourier Transform Infrared
Spleen cytology
Immunologic Factors pharmacology
Momordica charantia chemistry
Plant Extracts pharmacology
Polysaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 19
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 25178064
- Full Text :
- https://doi.org/10.3390/molecules190913432