A sentinel protein assay for simultaneously quantifying cellular processes.

Authors :: Soste M
Hrabakova R
Wanka S
Melnik A
Boersema P
Maiolica A
Wernas T
Tognetti M
von Mering C
Picotti P
Source :: Nature methods [Nat Methods] 2014 Oct; Vol. 11 (10), pp. 1045-8. Date of Electronic Publication: 2014 Sep 07.
Publication Year :: 2014
Abstract: We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.