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Direct involvement of CD56 in cytokine-induced killer-mediated lysis of CD56+ hematopoietic target cells.
- Source :
-
Experimental hematology [Exp Hematol] 2014 Dec; Vol. 42 (12), pp. 1013-21.e1. Date of Electronic Publication: 2014 Sep 06. - Publication Year :
- 2014
-
Abstract
- Cytokine-induced killer (CIK) cells are in-vitro-expanded T lymphocytes that represent a heterogeneous population. A large majority of CIK cells are CD3(+)CD56(+), and this population has been shown to confer a cytotoxic effect against tumor targets. The scope of this work was to study whether CD56 has a direct role in CIK-mediated cytotoxicity. Blocking of CD56 with the anti-CD56 monoclonal antibody GPR165 significantly reduced CIK-mediated lysis of three CD56(+) hematopoietic tumor cell lines (AML-NS8, NB4, and KCL22), whereas no effect was observed on three CD56(-) hematopoietic tumor cell lines (K562, REH, and MOLT-4). Knockdown of CD56 in CIK cells by short interfering RNA made the cells less cytotoxic against a CD56(+) target, and knockdown of CD56 in target cells with lentiviral short hairpin RNA significantly altered their susceptibility to CIK-mediated lysis. Our data suggest that homophilic interaction between CD56 molecules may occur in tumor-cell recognition, leading to CIK-mediated cell death.<br /> (Copyright © 2014 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Antibodies, Monoclonal pharmacology
CD56 Antigen chemistry
CD56 Antigen genetics
CD56 Antigen immunology
Cell Adhesion
Cell Line, Tumor
Cytotoxicity Tests, Immunologic
Cytotoxicity, Immunologic physiology
Electroporation
Gene Knockdown Techniques
Genetic Vectors genetics
Humans
Leukemia pathology
Protein Isoforms chemistry
Protein Isoforms genetics
Protein Isoforms physiology
RNA, Small Interfering pharmacology
Structure-Activity Relationship
CD56 Antigen physiology
Cytokine-Induced Killer Cells physiology
Hematopoietic Stem Cells
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2399
- Volume :
- 42
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Experimental hematology
- Publication Type :
- Academic Journal
- Accession number :
- 25201755
- Full Text :
- https://doi.org/10.1016/j.exphem.2014.08.005