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Total polysaccharide of Yupingfeng protects against bleomycin-induced pulmonary fibrosis via inhibiting transforming growth factor-β1-mediated type I collagen abnormal deposition in rats.
- Source :
-
The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2014 Dec; Vol. 66 (12), pp. 1786-95. Date of Electronic Publication: 2014 Sep 11. - Publication Year :
- 2014
-
Abstract
- Objective: This study was to explore the antifibrotic effect and the possible mechanism of total polysaccharides of Yupingfeng (YPF-P) on bleomycin (BLM)-induced pulmonary fibrosis in rats.<br />Methods: Pulmonary fibrosis was induced in Sprague-Dawley rats by BLM (5 mg/kg), killed 14 and 28 days after BLM administration by abdominal aorta exsanguination and removed the lungs. Lung coefficient was counted at the same time. Besides, H&E and Masson's trichrome staining for histopathological changes of lung tissues were observed. Additionally, western blotting and immunohistochemical staining techniques were used to detect expression of transforming growth factor-β1 (TGF-β1), type I collagen (Col-I) and α-smooth muscle actin (α-SMA). Finally, the levels of Col-I and hydroxyproline (HYP) in lung tissues were also utilized.<br />Key Findings: YPF-P alleviated the increase of lung coefficient induced by BLM instillation in pulmonary fibrosis rat, pathologic changes and collagen distribution were obviously ameliorated, while the increase of α-SMA-positive cells and TGF-β1 expression was prevented after YPF-P treatment. Moreover, the contents of HYP and Col-I were decreased in YPF-P group.<br />Conclusions: YPF-P had antifibrotic effect in experiment, which may reduce the synthesis and promote the deposition of Col-I via suppressing the increase of TGF-β1-mediated activation of myofibroblasts.<br /> (© 2014 Royal Pharmaceutical Society.)
- Subjects :
- Actins metabolism
Animals
Blotting, Western
Collagen Type I genetics
Disease Models, Animal
Drugs, Chinese Herbal administration & dosage
Drugs, Chinese Herbal chemistry
Hydroxyproline genetics
Hydroxyproline metabolism
Lung drug effects
Lung metabolism
Lung pathology
Polysaccharides administration & dosage
Polysaccharides chemistry
Pulmonary Fibrosis chemically induced
Pulmonary Fibrosis metabolism
Pulmonary Fibrosis pathology
Rats, Sprague-Dawley
Transforming Growth Factor beta1 genetics
Bleomycin pharmacology
Collagen Type I metabolism
Drugs, Chinese Herbal therapeutic use
Polysaccharides therapeutic use
Pulmonary Fibrosis prevention & control
Transforming Growth Factor beta1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2042-7158
- Volume :
- 66
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Journal of pharmacy and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 25209833
- Full Text :
- https://doi.org/10.1111/jphp.12308