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Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.
- Source :
-
PloS one [PLoS One] 2014 Sep 12; Vol. 9 (9), pp. e107354. Date of Electronic Publication: 2014 Sep 12 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- The facultative anaerobe, Bacillus cereus, causes diarrheal diseases in humans. Its ability to deal with oxygen availability is recognized to be critical for pathogenesis. The B. cereus genome comprises a gene encoding a protein with high similarities to the redox regulator, Rex, which is a central regulator of anaerobic metabolism in Bacillus subtilis and other Gram-positive bacteria. Here, we showed that B. cereus rex is monocistronic and down-regulated in the absence of oxygen. The protein encoded by rex is an authentic Rex transcriptional factor since its DNA binding activity depends on the NADH/NAD+ ratio. Rex deletion compromised the ability of B. cereus to cope with external oxidative stress under anaerobiosis while increasing B. cereus resistance against such stress under aerobiosis. The deletion of rex affects anaerobic fermentative and aerobic respiratory metabolism of B. cereus by decreasing and increasing, respectively, the carbon flux through the NADH-recycling lactate pathway. We compared both the cellular proteome and exoproteome of the wild-type and Δrex cells using a high throughput shotgun label-free quantitation approach and identified proteins that are under control of Rex-mediated regulation. Proteomics data have been deposited to the ProteomeXchange with identifier PXD000886. The data suggest that Rex regulates both the cross-talk between metabolic pathways that produce NADH and NADPH and toxinogenesis, especially in oxic conditions.
- Subjects :
- Anaerobiosis genetics
Bacillus cereus metabolism
DNA, Bacterial genetics
Enterotoxins biosynthesis
Enterotoxins metabolism
Exotoxins biosynthesis
Exotoxins genetics
Gene Expression Regulation, Bacterial
Gene Products, rex genetics
Humans
Membrane Proteins genetics
Metabolic Networks and Pathways genetics
Molecular Chaperones biosynthesis
Molecular Chaperones genetics
NAD metabolism
Protein Binding genetics
Bacillus cereus genetics
Gene Products, rex biosynthesis
Membrane Proteins biosynthesis
Proteomics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25216269
- Full Text :
- https://doi.org/10.1371/journal.pone.0107354