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Tumor necrosis factor alpha -238 G/A and -308 G/A polymorphisms and soluble TNF-α levels in chronic kidney disease: correlation with clinical variables.

Authors :
Vázquez-Huerta DI
Alvarez-Rodríguez BA
Topete-Reyes JF
Muñoz-Valle JF
Parra-Michel R
Fuentes-Ramírez F
Salazar-López MA
Valle Y
Reyes-Castillo Z
Cruz-González A
Brennan-Bourdon LM
Torres-Carrillo N
Source :
International journal of clinical and experimental medicine [Int J Clin Exp Med] 2014 Aug 15; Vol. 7 (8), pp. 2111-9. Date of Electronic Publication: 2014 Aug 15 (Print Publication: 2014).
Publication Year :
2014

Abstract

Chronic kidney disease (CKD) is characterized by accumulation of proinflammatory cytokines, mainly tumor necrosis factor alpha (TNF-α). Single nucleotide polymorphisms (SNPs) of TNFA gene, including -238 G/A and -308 G/A, have been associated with alteration in the soluble TNF-α (sTNF-α) expression. The aim was to investigate the association of -238 y -308 TNFA gene SNPs with sTNF-α levels in CKD patients. We included 150 CKD patients and 192 control subjects (CS). Both SNPs were genotyped with polymerase chain reaction-restriction fragment length polymorphism technique and sTNF-α levels were measured by enzyme-linked immunosorbent assay. The genotypic distribution of -238 and -308 SNPs was not significantly different between CKD patients and CS (p > 0.001). However, the sTNF-α levels were higher in CKD, compared to CS (p < 0.001). Also, sTNF-α correlated with creatinine (r = 0.279, p = 0.004), urea (r = 0.325, p = 0.001), phosphorus (r = 0.479, p = 0.001), glomerular filtration rate (r = -0.236, p = 0.019) and monocyte count (r = 0.276, p = 0.010). In conclusion, elevated sTNF-α levels are associated with CKD. However, the -238 and -308 TNFA gene SNPs were not associated with susceptibility to CKD and sTNF-α levels in a Mexican population.

Details

Language :
English
ISSN :
1940-5901
Volume :
7
Issue :
8
Database :
MEDLINE
Journal :
International journal of clinical and experimental medicine
Publication Type :
Academic Journal
Accession number :
25232395