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Genetic and structural analysis of Merkel cell polyomavirus large T antigen from diverse biological samples.
- Source :
-
Intervirology [Intervirology] 2014; Vol. 57 (6), pp. 331-6. Date of Electronic Publication: 2014 Sep 18. - Publication Year :
- 2014
-
Abstract
- Merkel cell polyomavirus (MCPyV) large T antigen (LT-ag) is frequently found truncated in Merkel cell carcinomas (MCC) and it is considered a major tumor-specific signature. Nonetheless, the biological role of LT-ag nontruncated mutations is largely unknown. In this study, MCPyV LT-ag second exon from 11 non-MCC oral samples and NCBI sequences derived from different anatomical sites were studied from the genetic and structural standpoint. As expected, the LT-ag mutation profile was influenced by the geographical origin of the sample, although nonsynonymous mutations were more frequent in lesional tissues. Our in silico study suggests that the mutations found would not significantly affect protein functions, regardless of sample category. This work presents a thorough investigation of the structural and functional properties of LT-ag nontruncated mutations in MCPyV. Our results sustain the geographical influence of the MCPyV genetic profile, but do not discard genetic tissue specificities. Further investigation involving other genetic segments in healthy and lesional tissues are necessary to improve our knowledge on MCPyV pathogenesis.<br /> (© 2014 S. Karger AG, Basel.)
- Subjects :
- Base Sequence
Computer Simulation
Exons genetics
Humans
Molecular Dynamics Simulation
Mutant Proteins chemistry
Mutant Proteins genetics
Mutation
Protein Structure, Tertiary
Saliva virology
Sequence Analysis, DNA
Skin virology
Antigens, Polyomavirus Transforming genetics
Antigens, Polyomavirus Transforming immunology
Carcinoma, Merkel Cell virology
Merkel cell polyomavirus immunology
Skin Neoplasms virology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0100
- Volume :
- 57
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Intervirology
- Publication Type :
- Academic Journal
- Accession number :
- 25247791
- Full Text :
- https://doi.org/10.1159/000363241