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The mechanisms involved in the activation of human natural killer cells by staphylococcal enterotoxin B.
- Source :
-
Cellular immunology [Cell Immunol] 1989 Aug; Vol. 122 (1), pp. 108-21. - Publication Year :
- 1989
-
Abstract
- The induction of enhanced natural cytotoxicity from human peripheral mononuclear cells by staphylococcal enterotoxin B (SEB) was examined. The activated killer cytotoxicity (AKC) was maximum at 16 hr with 1 mg/ml SEB. The precursor and effector cells of AKC were determined to be primarily CD5 negative, CD8 negative, CD16 positive cells. Monocytes and interleukin-1 played no role in the generation of AKC. However, a major role for interleukin-2 (IL-2) in AKC was shown by the inhibition of AKC when anti-IL-2 antibody or cyclosporin was added to the induction cultures. SEB rapidly induced the production of IL-2 from glass nonadherent cells by 6 hr and reached peak levels by 24 hr (162 U/ml). IL-2 induced by SEB in these induction cultures was preferentially produced by CD16 positive cells. Even though interferon-gamma (IFN-gamma) production was induced in these cultures, no role for IFN could be shown in SEB-induced AKC.
- Subjects :
- Antigens, Differentiation analysis
Cytotoxicity, Immunologic
Dose-Response Relationship, Drug
Humans
In Vitro Techniques
Interferon-gamma physiology
Interleukin-2 physiology
Killer Cells, Natural immunology
Phenotype
Receptors, Fc analysis
Receptors, IgG
Enterotoxins pharmacology
Killer Cells, Natural drug effects
Lymphocyte Activation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0008-8749
- Volume :
- 122
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 2526685
- Full Text :
- https://doi.org/10.1016/0008-8749(89)90152-4