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[Protective effect of rosmarinic acid on hypoxia/reoxygenation injury in cardiomyocytes].
- Source :
-
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2014 May; Vol. 39 (10), pp. 1897-901. - Publication Year :
- 2014
-
Abstract
- Objective: To study the protective effect of rosmarinic acid (Ros A) on the primary cardiomyocyte hypoxia/reoxygenation (H/R) injury.<br />Method: Primary cardiomyocytes of rats were cultured in vitro to establish the H/R injury of cardiomyocytes and observe the changes in the cell viability and LDH leakage. The changes in ATP content and ROS in cardiomyocytes were measured by using chemiluminescence and fluorescent probe technique. The effects of rosmarinic acid on the apoptosis of cardiomyocytes, cleaved-caspase 3, Akt and p-Akt protein expression were further detected by flow cytometry and western blot analysis.<br />Result: According to the experimental results, Ros A at doses of 25, 50, 100 mg x L(-1) could inhibit the decrease in H/R-induced cell viability, LDH leakage and excessive ROS generation, and maintain the ATP level in cells. Ros A at doses of 50, 100 mg x L(-1) could remarkably inhibit the H/R-induced cardiomyocyte apoptosis and down-regulate the expression of cleaved caspase-3. Moreover, Ros A at doses of 100 mg x L(-1) could significantly up-regulate the expression of p-Akt.<br />Conclusion: Ros A has the significant effect in resisting the cardiomyocyte H/R injury, improve cardiomyocyte energy metabolism and reduce cell apoptosis, which is related to the activation of Akt pathway.
- Subjects :
- Adenosine Triphosphate metabolism
Animals
Apoptosis drug effects
Caspase 3 metabolism
Cell Hypoxia drug effects
Cell Survival drug effects
Cells, Cultured
Humans
Hypoxia genetics
Hypoxia physiopathology
Hypoxia prevention & control
Male
Myocytes, Cardiac cytology
Myocytes, Cardiac metabolism
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species metabolism
Rosmarinic Acid
Cinnamates pharmacology
Depsides pharmacology
Hypoxia metabolism
Myocytes, Cardiac drug effects
Oxygen metabolism
Plant Extracts pharmacology
Protective Agents pharmacology
Rosmarinus chemistry
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-5302
- Volume :
- 39
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
- Publication Type :
- Academic Journal
- Accession number :
- 25282902