Back to Search
Start Over
Activation of the Nrf2-ARE pathway in hepatocytes protects against steatosis in nutritionally induced non-alcoholic steatohepatitis in mice.
- Source :
-
Toxicological sciences : an official journal of the Society of Toxicology [Toxicol Sci] 2014 Dec; Vol. 142 (2), pp. 361-74. Date of Electronic Publication: 2014 Oct 06. - Publication Year :
- 2014
-
Abstract
- Oxidative stress is implicated in the development of non-alcoholic steatohepatitis (NASH). The Nrf2-antioxidant response element pathway protects cells from oxidative stress. Studies have shown that global Nrf2 deficiency hastens the progression of NASH. The purpose of this study was to determine whether long-term hepatocyte-specific activation of Nrf2 mitigates NASH progression. Transgenic mice expressing a constitutively active Nrf2 construct in hepatocytes (AlbCre+/caNrf2+) and littermate controls were generated. These mice were fed standard or methionine-choline-deficient (MCD) diet, a diet used to induce NASH development in rodents. After 28 days of MCD dietary feeding, mice developed significant increases in steatosis, inflammation, oxidative stress, and HSC activation compared with those mice on standard diet. AlbCre+/caNrf2+ animals had significantly decreased serum transaminases and reduced steatosis when compared with the AlbCre+/caNrf2- animals. This significant reduction in steatosis was associated with increased expression of genes involved in triglyceride export (MTTP) and β-oxidation (CPT2). However, there were no differences in the increased oxidative stress, inflammation, and HSC activation from MCD diet administration between the AlbCre+/caNrf2- and AlbCre+/caNrf2+ animals. We conclude that hepatocyte-specific activation of Nrf2-mediated gene expression decreased hepatocellular damage and steatosis in a dietary model of NASH. However, hepatocyte-specific induction of Nrf2-mediated gene expression alone is insufficient to mitigate inflammation, oxidative stress, and HSC activation in this nutritional NASH model.<br /> (© The Author 2014. Published by Oxford University Press on behalf of the Society of Toxicology.All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Animals
Blotting, Western
Diet
Gene Expression physiology
Hepatocytes pathology
Immunohistochemistry
Lipid Metabolism genetics
Liver Function Tests
Mice, Transgenic
NF-E2-Related Factor 2 genetics
Non-alcoholic Fatty Liver Disease etiology
Non-alcoholic Fatty Liver Disease pathology
Non-alcoholic Fatty Liver Disease prevention & control
Promoter Regions, Genetic
Signal Transduction
Triglycerides metabolism
Antioxidant Response Elements genetics
Hepatocytes metabolism
NF-E2-Related Factor 2 metabolism
Non-alcoholic Fatty Liver Disease metabolism
Oxidative Stress
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0929
- Volume :
- 142
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Toxicological sciences : an official journal of the Society of Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 25294219
- Full Text :
- https://doi.org/10.1093/toxsci/kfu184