A rapid method for preparation of the cerebrospinal fluid proteome.

Authors :: Larssen E
Brede C
Hjelle AB
Øysaed KB
Tjensvoll AB
Omdal R
Ruoff P
Source :: Proteomics [Proteomics] 2015 Jan; Vol. 15 (1), pp. 10-5. Date of Electronic Publication: 2014 Dec 03.
Publication Year :: 2015
Abstract: The cerebrospinal fluid (CSF) proteome is of great interest for investigation of diseases and conditions involving the CNS. However, the presence of high-abundance proteins (HAPs) can interfere with the detection of low-abundance proteins, potentially hindering the discovery of new biomarkers. Therefore, an assessment of the CSF subproteome composition requires depletion strategies. Existing methods are time consuming, often involving multistep protocols. Here, we present a rapid, accurate, and reproducible method for preparing the CSF proteome, which allows the identification of a high number of proteins. This method involves acetonitrile (ACN) precipitation for depleting HAPs, followed by immediate trypsination. As an example, we demonstrate that this method allows discrimination between multiple sclerosis patients and healthy subjects.<br /> (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)