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Salicylic acid inhibits enzymatic browning of fresh-cut Chinese chestnut (Castanea mollissima) by competitively inhibiting polyphenol oxidase.

Authors :
Zhou D
Li L
Wu Y
Fan J
Ouyang J
Source :
Food chemistry [Food Chem] 2015 Mar 15; Vol. 171, pp. 19-25. Date of Electronic Publication: 2014 Sep 04.
Publication Year :
2015

Abstract

The inhibitory effect and associated mechanisms of salicylic acid (SA) on the browning of fresh-cut Chinese chestnut were investigated. Shelled and sliced chestnuts were immersed in different concentrations of an SA solution, and the browning of the chestnut surface and interior were inhibited. The activities of polyphenol oxidase (PPO) and peroxidase (POD) extracted from chestnuts were measured in the presence and absence of SA. SA at concentrations higher than 0.3g/L delayed chestnut browning by significantly inhibiting the PPO activity (P<0.01), and the POD activity was not significantly affected (P>0.05). The binding and inhibition modes of SA with PPO and POD, determined by AUTODOCK 4.2 and Lineweaver-Burk plots, respectively, established SA as a competitive inhibitor of PPO.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1873-7072
Volume :
171
Database :
MEDLINE
Journal :
Food chemistry
Publication Type :
Academic Journal
Accession number :
25308637
Full Text :
https://doi.org/10.1016/j.foodchem.2014.08.115