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Elatoside C protects against hypoxia/reoxygenation-induced apoptosis in H9c2 cardiomyocytes through the reduction of endoplasmic reticulum stress partially depending on STAT3 activation.
- Source :
-
Apoptosis : an international journal on programmed cell death [Apoptosis] 2014 Dec; Vol. 19 (12), pp. 1727-35. - Publication Year :
- 2014
-
Abstract
- Endoplasmic reticulum (ER) stress-induced apoptosis has been suggested to contribute to myocardial ischemia-reperfusion (I/R) injury. Elatoside C is one of the major triterpenoid compounds isolated from Aralia elata that is known to be cardioprotective. However, its effects on I/R injury to cardiac myocytes have not been clarified. This study aimed to investigate the possible protective effect of Elatoside C against hypoxia/reoxygenation (H/R)-induced H9c2 cardiomyocyte injury and its underlying mechanisms. H9c2 cardiomyocytes were subjected to H/R in the presence of Elatoside C. Our results showed that Elatoside C (25 μM) treatment provided significant protection against H/R-induced cell death, as evidenced by improved cell viability, maintained mitochondrial membrane potential, diminished mitochondrial ROS, and reduced apoptotic cardiomyocytes (P < 0.05). These changes were associated with the inhibition of ER stress-associated apoptosis markers (GRP78, CHOP, Caspase-12 and JNK), as well as the increased phosphorylation of STAT3 and an increased Bcl2/Bax ratio. Moreover, these effects of Elatoside C were prevented by the STAT3 inhibitor Stattic. Taken together, these results suggested that Elatoside C can alleviate H/R-induced cardiomyocyte apoptosis most likely by activating the STAT3 pathways and reducing ER stress-associated apoptosis.
- Subjects :
- Animals
Aralia
Cell Hypoxia
Cell Line
Myocardial Reperfusion Injury metabolism
Myocardial Reperfusion Injury pathology
Myocytes, Cardiac metabolism
Myocytes, Cardiac pathology
Rats
Apoptosis drug effects
Cardiotonic Agents pharmacology
Endoplasmic Reticulum Stress drug effects
Myocytes, Cardiac drug effects
STAT3 Transcription Factor metabolism
Saponins pharmacology
Triterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-675X
- Volume :
- 19
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Apoptosis : an international journal on programmed cell death
- Publication Type :
- Academic Journal
- Accession number :
- 25326083
- Full Text :
- https://doi.org/10.1007/s10495-014-1039-3