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Protective efficacy of a Plasmodium vivax circumsporozoite protein-based vaccine in Aotus nancymaae is associated with antibodies to the repeat region.

Authors :
Yadava A
Hall CE
Sullivan JS
Nace D
Williams T
Collins WE
Ockenhouse CF
Barnwell JW
Source :
PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2014 Oct 16; Vol. 8 (10), pp. e3268. Date of Electronic Publication: 2014 Oct 16 (Print Publication: 2014).
Publication Year :
2014

Abstract

We have previously reported that Vivax Malaria Protein 001 (VMP001), a vaccine candidate based on the circumsporozoite protein of Plasmodium vivax, is immunogenic in mice and rhesus monkeys in the presence of various adjuvants. In the present study, we evaluated the immunogenicity and efficacy of VMP001 formulated with a TLR9 agonist in a water-in-oil emulsion. Following immunization, the vaccine efficacy was assessed by challenging Aotus nancymaae monkeys with P. vivax sporozoites. Monkeys from both the low- and high-dose vaccine groups generated strong humoral immune responses to the vaccine (peak median titers of 291,622), and its subunits (peak median titers to the N-term, central repeat and C-term regions of 22,188; 66,120 and 179,947, respectively). 66.7% of vaccinated monkeys demonstrated sterile protection following challenge. Protection was associated with antibodies directed against the central repeat region. The protected monkeys had a median anti-repeat titer of 97,841 compared to 14,822 in the non-protected monkeys. This is the first report demonstrating P. vivax CSP vaccine-induced protection of Aotus monkeys challenged with P. vivax sporozoites.

Details

Language :
English
ISSN :
1935-2735
Volume :
8
Issue :
10
Database :
MEDLINE
Journal :
PLoS neglected tropical diseases
Publication Type :
Academic Journal
Accession number :
25329054
Full Text :
https://doi.org/10.1371/journal.pntd.0003268