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Wisteria floribunda agglutinin-binding glycan expression is decreased in endometriomata.
- Source :
-
Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2014 Oct 24; Vol. 12, pp. 100. Date of Electronic Publication: 2014 Oct 24. - Publication Year :
- 2014
-
Abstract
- Background: Glycosylation is one of the most common post-translational modifications of eukaryotic proteins and is known to undergo dynamic changes in a wide range of biological processes. To date, however, the glycan expression profiles in endometriosis are largely unknown. The objective of the study was to identify the panel of glycans that were aberrantly expressed in endometriosis, a hormone-dependent disease.<br />Methods: The glycan expression profiles in primary cultured human endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs) were determined by lectin microarray analysis. Distribution of Wisteria floribunda agglutinin (WFA)-binding glycans in ovarian endometriotic cysts and eutopic proliferative phase endometrium were assessed by lectin histochemistry. The expressions of N-acetylgalactosaminyl transferases that synthesize WFA-binding glycans were evaluated in ECSCs and NESCs.<br />Results: We found that the levels of WFA-binding glycans were decreased in ECSCs. Lectin histochemistry revealed that WFA-binding glycans were decreased only in the stromal components of the ovarian endometriotic cysts, but not in the epithelial components, compared to the eutopic proliferative phase endometrium. The expressions of N-acetylgalactosaminyl transferases that synthesize WFA-binding glycans were downregulated in ECSCs.<br />Conclusions: Utilizing lectin microarray analysis and lectin histochemistry, we found that WFA-binding glycans were decreased in endometriosis. The synthetic enzymes of WFA-binding glycans were significantly downregulated in ECSCs. It is suggested that reduced expression of N-glycans with WFA-binding properties on ECSCs is a novel characteristics of endometriosis.
- Subjects :
- Adult
Blotting, Western
Cells, Cultured
Endometriosis enzymology
Endometriosis pathology
Endometrium enzymology
Endometrium metabolism
Endometrium pathology
Female
Follicular Phase
Humans
Isoenzymes genetics
Isoenzymes metabolism
N-Acetylgalactosaminyltransferases genetics
Ovarian Cysts enzymology
Ovarian Cysts pathology
Ovary enzymology
Ovary metabolism
Ovary pathology
Plant Lectins antagonists & inhibitors
Polysaccharides metabolism
Protein Array Analysis
RNA, Messenger metabolism
Receptors, N-Acetylglucosamine antagonists & inhibitors
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells enzymology
Stromal Cells metabolism
Stromal Cells pathology
Down-Regulation
Endometriosis metabolism
N-Acetylgalactosaminyltransferases metabolism
Ovarian Cysts metabolism
Plant Lectins metabolism
Polysaccharides biosynthesis
Receptors, N-Acetylglucosamine metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1477-7827
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Reproductive biology and endocrinology : RB&E
- Publication Type :
- Academic Journal
- Accession number :
- 25344456
- Full Text :
- https://doi.org/10.1186/1477-7827-12-100