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UV-B-responsive association of the Arabidopsis bZIP transcription factor ELONGATED HYPOCOTYL5 with target genes, including its own promoter.
- Source :
-
The Plant cell [Plant Cell] 2014 Oct; Vol. 26 (10), pp. 4200-13. Date of Electronic Publication: 2014 Oct 28. - Publication Year :
- 2014
-
Abstract
- In plants subjected to UV-B radiation, responses are activated that minimize damage caused by UV-B. The bZIP transcription factor ELONGATED HYPOCOTYL5 (HY5) acts downstream of the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8) and promotes UV-B-induced photomorphogenesis and acclimation. Expression of HY5 is induced by UV-B; however, the transcription factor(s) that regulate HY5 transcription in response to UV-B and the impact of UV-B on the association of HY5 with its target promoters are currently unclear. Here, we show that HY5 binding to the promoters of UV-B-responsive genes is enhanced by UV-B in a UVR8-dependent manner in Arabidopsis thaliana. In agreement, overexpression of REPRESSOR OF UV-B PHOTOMORPHOGENESIS2, a negative regulator of UVR8 function, blocks UV-B-responsive HY5 enrichment at target promoters. Moreover, we have identified a T/G-box in the HY5 promoter that is required for its UV-B responsiveness. We show that HY5 and its homolog HYH bind to the T/G(HY5)-box cis-acting element and that they act redundantly in the induction of HY5 expression upon UV-B exposure. Therefore, HY5 is enriched at target promoters in response to UV-B in a UVR8 photoreceptor-dependent manner, and HY5 and HYH interact directly with a T/G-box cis-acting element of the HY5 promoter, mediating the transcriptional activation of HY5 in response to UV-B.<br /> (© 2014 American Society of Plant Biologists. All rights reserved.)
- Subjects :
- Arabidopsis genetics
Arabidopsis growth & development
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Basic-Leucine Zipper Transcription Factors metabolism
Binding Sites genetics
Carrier Proteins genetics
Carrier Proteins metabolism
Chromosomal Proteins, Non-Histone metabolism
DNA-Binding Proteins
Gene Expression Regulation, Developmental radiation effects
Gene Expression Regulation, Plant radiation effects
Morphogenesis genetics
Morphogenesis radiation effects
Mutation
Nuclear Proteins metabolism
Plants, Genetically Modified
Protein Binding
Regulatory Sequences, Nucleic Acid genetics
Reverse Transcriptase Polymerase Chain Reaction
Arabidopsis Proteins genetics
Basic-Leucine Zipper Transcription Factors genetics
Chromosomal Proteins, Non-Histone genetics
Nuclear Proteins genetics
Promoter Regions, Genetic genetics
Ultraviolet Rays
Subjects
Details
- Language :
- English
- ISSN :
- 1532-298X
- Volume :
- 26
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 25351492
- Full Text :
- https://doi.org/10.1105/tpc.114.130716