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Analysis of heparanase isoforms and cathepsin B in the plasma of patients with gastrointestinal carcinomas: analytical cross-sectional study.

Authors :
Melo CM
Origassa CS
Theodoro TR
Matos LL
Miranda TA
Accardo CM
Bouças RI
Suarez ER
Pares MM
Waisberg DR
Toloi GC
Nader HB
Waisberg J
Pinhal MA
Source :
Sao Paulo medical journal = Revista paulista de medicina [Sao Paulo Med J] 2015 Feb; Vol. 133 (1), pp. 28-35.
Publication Year :
2015

Abstract

Context and Objective: Heparanase-1 degrades heparan sulfate and has been correlated with tumor progression. Although the isoform heparanase-2 has no catalytic activity, it seems to be important for modulating heparanase-1 activity. Cathepsin B is a proteinase involved in tumor metastasis. The aim of this study was to analyze heparanase isoform expression and cathepsin B activity in plasma samples from patients with gastrointestinal carcinomas, compared with healthy individuals (control group).<br />Design and Setting: This was an analytical cross-sectional study. Peripheral blood samples were collected at a Brazilian public hospital, from 21 patients with histopathological diagnoses of gastrointestinal carcinomas and from 43 healthy individuals. The analyses were performed in two Brazilian medical schools.<br />Methods: Heparanase isoforms were identified and quantified in plasma samples by means of Western blot. The enzymatic activities of heparanase-1 and cathepsin B were also measured.<br />Results: The results demonstrated that the expression of both heparanase isoforms was significantly greater in plasma samples from gastrointestinal carcinoma patients, compared with the control group. Logistic regression analysis showed that increased heparanase-1 and heparanase-2 expression was exclusively dependent on the tumor. There was a significant increase in heparanase-1 and cathepsin B activity in the patients' plasma.<br />Conclusion: Overexpression of heparanase-1 and heparanase-2, along with increased heparanase-1 and cathepsin B activity in plasma, is associated with the diagnosis of gastrointestinal carcinoma. These findings provide support for using non-invasive assays (plasma samples) as an auxiliary method for diagnosing gastrointestinal tumors.

Details

Language :
English
ISSN :
1806-9460
Volume :
133
Issue :
1
Database :
MEDLINE
Journal :
Sao Paulo medical journal = Revista paulista de medicina
Publication Type :
Academic Journal
Accession number :
25351637
Full Text :
https://doi.org/10.1590/1516-3180.2013.7080003