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[Physical characterization of decellularized cartilage matrix for reconstructive rhinosurgery].
- Source :
-
Laryngo- rhino- otologie [Laryngorhinootologie] 2014 Nov; Vol. 93 (11), pp. 756-63. Date of Electronic Publication: 2014 Nov 04. - Publication Year :
- 2014
-
Abstract
- Background: The use of autologous auricular and rib cartilage for the reconstruction of nasal defects and deformities is associated with a number of disadvantages. The development of alternative materials is therefore the focus of intensive research. Recent studies demonstrated that decellularized cartilage is a promising material for cartilage tissue engineering. Hence, the aim of this study was to characterize the materials surface and cellular reactions to the decellularized cartilage matrix in long term-3D-culture.<br />Material and Methods: Material geometry of decellularized cartilage was examined by microcomputed tomography as well as material characteristics by scanning and transmission electron microscopy. The expression of integrins on the surface of human chondrocytes was determined after seeding and migration into the scaffold.<br />Results: After decellularization an obvious enlargement of the matrix surface and an intensive interaction between the chondrocytes and the collagen matrix was observed. ITGA1 and ITGB1 were upregulated indicating chondrogenic differentiation.<br />Conclusion: Therefore, decellularized porcine cartilage provides an optimal microstructure for human chondrocytes with respect to cell integration and matrix production. Thus, it offers promising characteristics for clinical application in reconstructive surgery.<br /> (© Georg Thieme Verlag KG Stuttgart · New York.)
- Subjects :
- Cartilage chemistry
Cell Differentiation physiology
Cell Movement physiology
Chondrocytes chemistry
Chondrocytes transplantation
Chondrocytes ultrastructure
Humans
Matrilin Proteins analysis
Tissue Engineering
Tissue Scaffolds
Cartilage transplantation
Cartilage ultrastructure
Matrilin Proteins therapeutic use
Matrilin Proteins ultrastructure
Microscopy, Electron, Scanning Transmission
Rhinoplasty methods
X-Ray Microtomography
Subjects
Details
- Language :
- German
- ISSN :
- 1438-8685
- Volume :
- 93
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Laryngo- rhino- otologie
- Publication Type :
- Academic Journal
- Accession number :
- 25369160
- Full Text :
- https://doi.org/10.1055/s-0034-1384531