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MiR-199a inhibits the ability of proliferation and migration by regulating CD44-Ezrin signaling in cutaneous squamous cell carcinoma cells.
- Source :
-
International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2014 Sep 15; Vol. 7 (10), pp. 7131-41. Date of Electronic Publication: 2014 Sep 15 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Cutaneous squamous cell carcinoma (cSCC), the second most common form of human cancer, is an epithelial skin tumor, which can result in metastasis with lethal consequences accounting for about 20% of all skin cancer-related deaths. The metastasis is the main reason for cSCC-related deaths with an overall 5-year survival rate < 30% in cases that spread systemically. The role of miRNAs has been involved in SCC of different origins. Recent data have revealed that the expression of miRNA-199a was changed in many human cancers. In this study, we found that miR-199a was significantly decreased in cSCC tissues, which had an inverse relationship with CD44. MiR-199a specifically regulated the expression of CD44 at mRNA and protein levels, and the interaction between CD44 and Ezrin in cSCC cells. Moreover, the suppressive role of miR-199a in cell migration in cSCC cells was also associated with the activity of MMP2 and MMP9. Taken together, our data indicated that increased expression of endogenous mature miR-199a might prevent the growth and migration of human cSCC via decreasing the expression of CD44 and regulating the interaction between CD44 and Ezrin, which may provide a potentially important therapeutic target for human cSCC.
- Subjects :
- 3' Untranslated Regions
Carcinoma, Squamous Cell genetics
Carcinoma, Squamous Cell pathology
Cell Line, Tumor
Cytoskeletal Proteins genetics
Gene Expression Regulation, Neoplastic
Humans
Hyaluronan Receptors genetics
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 metabolism
MicroRNAs genetics
Neoplasm Invasiveness
RNA, Messenger metabolism
Signal Transduction
Skin Neoplasms genetics
Skin Neoplasms pathology
Time Factors
Transfection
Carcinoma, Squamous Cell metabolism
Cell Movement
Cell Proliferation
Cytoskeletal Proteins metabolism
Hyaluronan Receptors metabolism
MicroRNAs metabolism
Skin Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1936-2625
- Volume :
- 7
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of clinical and experimental pathology
- Publication Type :
- Academic Journal
- Accession number :
- 25400809