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Redox-responsive branched-bottlebrush polymers for in vivo MRI and fluorescence imaging.
- Source :
-
Nature communications [Nat Commun] 2014 Nov 18; Vol. 5, pp. 5460. Date of Electronic Publication: 2014 Nov 18. - Publication Year :
- 2014
-
Abstract
- Stimuli-responsive multimodality imaging agents have broad potential in medical diagnostics. Herein, we report the development of a new class of branched-bottlebrush polymer dual-modality organic radical contrast agents--ORCAFluors--for combined magnetic resonance and near-infrared fluorescence imaging in vivo. These nitroxide radical-based nanostructures have longitudinal and transverse relaxation times that are on par with commonly used heavy-metal-based magnetic resonance imaging (MRI) contrast agents. Furthermore, these materials display a unique compensatory redox response: fluorescence is partially quenched by surrounding nitroxides in the native state; exposure to ascorbate or ascorbate/glutathione leads to nitroxide reduction and a concomitant 2- to 3.5-fold increase in fluorescence emission. This behaviour enables correlation of MRI contrast, fluorescence intensity and spin concentration with tissues known to possess high concentrations of ascorbate in mice. Our in vitro and in vivo results, along with our modular synthetic approach, make ORCAFluors a promising new platform for multimodality molecular imaging.
- Subjects :
- Animals
Ascorbic Acid chemistry
Contrast Media chemical synthesis
Female
Fluorescence
HeLa Cells
Humans
Mice
Mice, Inbred BALB C
Nitrogen Oxides chemistry
Oxidation-Reduction
Polymers chemical synthesis
Contrast Media chemistry
Magnetic Resonance Imaging instrumentation
Molecular Imaging instrumentation
Polymers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 25403521
- Full Text :
- https://doi.org/10.1038/ncomms6460