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Long non-coding RNA HOTAIR is associated with human cervical cancer progression.
- Source :
-
International journal of oncology [Int J Oncol] 2015 Feb; Vol. 46 (2), pp. 521-30. Date of Electronic Publication: 2014 Nov 17. - Publication Year :
- 2015
-
Abstract
- The functions of many long non-coding RNAs (lncRNAs) in human cancers remain to be clarified. The lncRNA Hox transcript antisense intergenic RNA (HOTAIR) has been reported to reprogram chromatin organization and promote breast and colorectal cancer metastasis, the involvement of lncRNAs in cervical cancer is just beginning to be studied. In the present study, we examined the expression and the functional role of HOTAIR in cervical cancer. HOTAIR expression was determined in cervical cancer tissues (n=111) and corresponding normal tissues (n=40) by using real-time polymerase chain reaction, and its correlation with clinical parameters and prognosis were analyzed. To determine the effect of HOTAIR knockdown and overexpression in cervical cancer cell lines, we used the CCK-8 assay, wound healing migration and matrigel invasion assay. The expression level of HOTAIR in cervical cancer tissues was higher than that in corresponding non-cancerous tissues. High HOTAIR expression correlated with lymph node metastasis, and reduced overall survival. A multivariate analysis showed that HOTAIR was a prognostic factor for predicting cervical cancer recurrence. Knockdown of HOTAIR reduced cell proliferation, migration, and invasion in cervical cancer cell lines. Moreover, HOTAIR regulated the expression of vascular endothelial growth factor, matrix metalloproteinase-9 and epithelial-to-mesenchymal transition (EMT)-related genes, which are important for cell motility and metastasis. Therefore, HOTAIR may promote tumor aggressiveness through the upregulation of VEGF and MMP-9 and EMT-related genes. These findings indicate that HOTAIR may represent a novel biomarker for predicting recurrence and prognosis and serve as a promising therapeutic target in cervical cancer.
- Subjects :
- Disease Progression
Epithelial-Mesenchymal Transition genetics
Female
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
Lymphatic Metastasis
Middle Aged
Neoplasm Recurrence, Local pathology
Neoplasm Staging
RNA, Long Noncoding genetics
Uterine Cervical Neoplasms pathology
Neoplasm Recurrence, Local genetics
Prognosis
RNA, Long Noncoding biosynthesis
Uterine Cervical Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 46
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 25405331
- Full Text :
- https://doi.org/10.3892/ijo.2014.2758