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Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B.
- Source :
-
BMB reports [BMB Rep] 2015 Jul; Vol. 48 (7), pp. 401-6. - Publication Year :
- 2015
-
Abstract
- Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed at the promoter area of ANGPT1. Using MTT and wound healing assays, depletion of KDM3B was found to increase cell proliferation and cell motility, indicating that KDM3B has a role in angiogenesis.
- Subjects :
- Angiopoietin-1 metabolism
Cell Movement
Cell Proliferation
Co-Repressor Proteins metabolism
Gene Expression Regulation
HCT116 Cells
Humans
K562 Cells
MCF-7 Cells
Nuclear Receptor Co-Repressor 2 metabolism
Protein Binding
Angiopoietin-1 genetics
Histones metabolism
Jumonji Domain-Containing Histone Demethylases metabolism
Lysine metabolism
Repressor Proteins metabolism
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1976-670X
- Volume :
- 48
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- BMB reports
- Publication Type :
- Academic Journal
- Accession number :
- 25413303
- Full Text :
- https://doi.org/10.5483/bmbrep.2015.48.7.188