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Identification of lipase encoding genes from Antarctic seawater bacteria using degenerate primers: expression of a cold-active lipase with high specific activity.
- Source :
-
Enzyme and microbial technology [Enzyme Microb Technol] 2015 Jan; Vol. 68, pp. 56-61. Date of Electronic Publication: 2014 Oct 25. - Publication Year :
- 2015
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Abstract
- Cold-active enzymes are valuable catalysts showing high activity at low and moderate temperatures and low thermostability. Among cold-active enzymes, lipases offer a great potential in detergent, cosmetic, biofuel and food or feed industries. In this paper we describe the identification of novel lipase coding genes and the expression of a lipase with high activity at low temperatures. The genomic DNA from Antarctic seawater bacteria showing lipolytic activity at 4°C was used to amplify five DNA fragments that partially encode novel lipases using specifically designed COnsensus-DEgenerate Hybrid Oligonucleotide Primers (CODEHOP). All the fragments were found to have a high identity with an α/β-hydrolase domain-containing protein identified by the sequencing of the complete genome of Shewanella frigidimarina NCIMB 400. The complete sequence of one of the lipase-coding gene fragments, lipE13, was obtained by genome walking. Considering that the other fragments had a high identity to the putative lipase from S. frigidimarina NCIMB 400, the complete lipase genes were amplified using oligonucleotide primers designed based on the 5' and 3' regions of the coding sequence of the related protein. This strategy allowed the amplification of 3 lipase-encoding genes of which one was expressed in the periplasm using the Escherichia coli BL21(DE3)/pET-22b(+) expression system. The recombinant protein was obtained with activity toward p-nitrophenyl caproate showing a high specific activity between 15 and 25°C.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Antarctic Regions
Caproates metabolism
Cold Temperature
DNA Primers
DNA, Bacterial isolation & purification
Escherichia coli metabolism
Phylogeny
Recombinant Fusion Proteins metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Shewanella enzymology
Shewanella genetics
Substrate Specificity
DNA, Bacterial genetics
Genes, Bacterial
Lipase genetics
Seawater microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0909
- Volume :
- 68
- Database :
- MEDLINE
- Journal :
- Enzyme and microbial technology
- Publication Type :
- Academic Journal
- Accession number :
- 25435506
- Full Text :
- https://doi.org/10.1016/j.enzmictec.2014.10.004