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Avidin-biotin capped mesoporous silica nanoparticles as an ion-responsive release system to determine lead(II).
- Source :
-
Analytical biochemistry [Anal Biochem] 2015 Feb 15; Vol. 471, pp. 17-22. Date of Electronic Publication: 2014 Nov 10. - Publication Year :
- 2015
-
Abstract
- We have developed DNAzyme-functionalized silica nanoparticles for the rapid, sensitive, and selective detection of lead ion (Pb(2+)). The specific binding between avidin and biotinylated DNAzymes was used to cap the pore of dye-trapped silica nanoparticles. In the presence of Pb(2+), DNAzymes were catalytically cleaved to uncap the pore, releasing the dye cargo with detectable enhancements of fluorescence signal. This method enables rapid (15 min) and sensitive (limit of detection=8.0 nM) detection. Moreover, the Pb(2+)-responsive behavior shows high selectivity with other metal ions. The superior properties of the as-designed DNAzyme-functionalized silica nanoparticles can be attributed to the large loading capacity and highly ordered pore structure of mesoporous silica nanoparticles as well as the catalytical cleaving of DNAzymes with Pb(2+). The recoveries obtained by standard Pb(II) addition to real samples-tap water, commercial mineral water, and lake water-were all from 98 to 101%. Our design serves as a new prototype for metal-ion sensing systems, and it also has promising potential for detection of various targets in stimulus-release systems.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Avidin metabolism
Base Sequence
Biocatalysis
Biotin metabolism
Coloring Agents chemistry
DNA, Catalytic genetics
DNA, Catalytic metabolism
Lead chemistry
Porosity
Water chemistry
Avidin chemistry
Biosensing Techniques methods
Biotin chemistry
Drug Liberation
Lead analysis
Nanoparticles chemistry
Silicon Dioxide chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0309
- Volume :
- 471
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25447495
- Full Text :
- https://doi.org/10.1016/j.ab.2014.10.020