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Studies on the interaction of apigenin with calf thymus DNA by spectroscopic methods.
- Source :
-
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2015 Feb 05; Vol. 136 Pt C, pp. 1666-70. Date of Electronic Publication: 2014 Oct 24. - Publication Year :
- 2015
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Abstract
- The interaction between apigenin and calf thymus deoxyribonucleic acid (ctDNA) in a pH 7.4 Tris-HCl buffer solution was investigated by UV-Vis spectroscopy, fluorescence spectroscopy, DNA melting techniques, and viscosity measurements. It was found that apigenin molecules could intercalate into the base pairs of DNA, forming a apigenin-DNA complex with a binding constant of K310K=6.4×10(4)Lmol(-1). The thermodynamic parameters enthalpy change (ΔH), entropy change (ΔS) and Gibbs free energy (ΔG) were calculated to be 7.36×10(4)Jmol(-1), 329JK(-1)mol(-1) and -2.84×10(4)Jmol(-1) at 310K, respectively. Hydrophobic interaction was the predominant intermolecular force in stabilizing the apigenin-DNA complex. Thermal denaturation study suggested that the stabilization of the ctDNA helix was increased when the apigenin binding to ctDNA as indicated by the increase in thermal denaturation temperature of ctDNA at around 5.0°C in the presence of apigenin. Spectroscopic techniques together with melting techniques and viscosity determination provided evidences of intercalation mode of binding for the interaction between apigenin and ctDNA.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Apigenin chemistry
Binding Sites
Circular Dichroism
DNA chemistry
DNA drug effects
Entropy
Intercalating Agents chemistry
Intercalating Agents pharmacokinetics
Nucleic Acid Denaturation
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Temperature
Thermodynamics
Viscosity
Apigenin pharmacokinetics
DNA metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3557
- Volume :
- 136 Pt C
- Database :
- MEDLINE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 25459730
- Full Text :
- https://doi.org/10.1016/j.saa.2014.10.062