PAMAM dendrimer with 4-carbomethoxypyrrolidone--in vitro assessment of neurotoxicity.

Authors :: Janaszewska A
Studzian M
Petersen JF
Ficker M
Christensen JB
Klajnert-Maculewicz B
Source :: Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2015 Feb; Vol. 11 (2), pp. 409-11. Date of Electronic Publication: 2014 Nov 14.
Publication Year :: 2015
Abstract: Cytotoxicity of cationic amino-terminated PAMAM dendrimer and modified PAMAM-pyrrolidone dendrimer was compared. LDH assay and cell visualization technique were employed. Mouse embryonic hippocampal cells (mHippoE-18) were used. The experiments were performed in FBS-deprived medium. Pyrrolidone-modification significantly diminished toxicity of PAMAM dendrimer. The absence of FBS did not reveal significant impact on the toxic effect. Results from LDH assay and MTT test were in good consistency. Low cytotoxicity of PAMAM-pyrrolidone dendrimer increases reliability of the results showing a small impact of this dendrimer on cell viability.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)

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