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ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: cloned and characterized in Crassostrea hongkongensis.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2015 Feb; Vol. 42 (2), pp. 225-32. Date of Electronic Publication: 2014 Nov 20. - Publication Year :
- 2015
-
Abstract
- Apoptosis has been primarily investigated in mammals, and little is known about apoptosis in mollusks. The proteins Bax and Bak play critical roles in the mitochondrial apoptosis pathway and in determining cell fate. In this study, ChBax and ChBak, homologs of the well-known Bax and Bak proteins, were identified from the oyster Crassostrea hongkongensis. The ChBax/Bak proteins consist of 207/232 amino acids with the typical domains found in BCL-2 family members. ChBax and ChBak mRNA expression were detected in all 8 of the selected oyster tissues and at the different stages of development. Fluorescence microscopy revealed that the full-length proteins of ChBax/Bak were located in the cytoplasm and mitochondrial outer membrane, of HEK293T cells, respectively. Furthermore, both of the genes' expression levels were found to increase in the hemocytes of oysters challenged with pathogens. The over-expression of ChBax or ChBak activates the p53-Luc reporter gene in HEK293T cells in a dose-dependent manner. These results indicate that ChBax and ChBak may play important roles in the mitochondrial apoptotic pathway in oysters.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Crassostrea metabolism
DNA, Complementary genetics
DNA, Complementary metabolism
Genes, Reporter
HEK293 Cells
Hemocytes metabolism
Humans
Mitochondria physiology
Molecular Sequence Data
Phylogeny
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Alignment
Tumor Suppressor Protein p53 genetics
Tumor Suppressor Protein p53 metabolism
bcl-2 Homologous Antagonist-Killer Protein metabolism
bcl-2-Associated X Protein metabolism
Apoptosis genetics
Crassostrea genetics
bcl-2 Homologous Antagonist-Killer Protein genetics
bcl-2-Associated X Protein genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 42
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25463302
- Full Text :
- https://doi.org/10.1016/j.fsi.2014.11.013