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Increased expression of endocan in arthritic synovial tissues: effects of adiponectin on the expression of endocan in fibroblast-like synoviocytes.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2015 Apr; Vol. 11 (4), pp. 2695-702. Date of Electronic Publication: 2014 Dec 05. - Publication Year :
- 2015
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Abstract
- This study was performed to evaluate whether endocan expression, which is known to be involved in tumor angiogenesis, was increased in rheumatoid arthritic tissues. In addition, the involvement of adiponectin in the regulation of endocan expression in arthritic joints was examined. Arthritic synovial tissues from patients with rheumatoid arthritis (RA) or osteoarthritis (OA) were immunostained with antibodies to endocan and vascular endothelial growth factor (VEGF). Subsequently, synovial cells and human umbilical vein endothelial cells were cultured and stimulated with interleukin-1 β (IL-1β) or adiponectin. The mRNA and protein levels of endocan were evaluated by polymerase chain reaction and ELISA, respectively. Endocan expression was markedly increased in the inflammatory sites of RA synovial tissues. In OA tissues, endocan expression was higher in tissues displaying moderate and severe inflammation than in those with mild inflammation. In vitro expression levels of endocan and VEGF in endothelial and synovial cells were differentially increased in response to IL-1β stimulation. Adiponectin was a more potent stimulant of endocan than IL-1β at their respective physiological concentrations in synovial cells. Endocan silencing by small interfering RNA transfection of synovial cells decreased in vitro cell migration and invasion. In conclusion, adiponectin is an important factor in the stimulation of endocan expression in synovial cells. Adiponectin-induced endocan expression in synovial cells may stimulate cell migration and invasion as well as angiogenesis in the pannus of arthritic joints.
- Subjects :
- Adiponectin metabolism
Adiponectin pharmacology
Arthritis metabolism
Arthritis pathology
Arthritis, Rheumatoid genetics
Arthritis, Rheumatoid metabolism
Arthritis, Rheumatoid pathology
Cell Movement drug effects
Endothelial Cells drug effects
Endothelial Cells metabolism
Fibroblasts drug effects
Fibroblasts metabolism
Gene Knockdown Techniques
Humans
Immunohistochemistry
Neoplasm Proteins metabolism
Osteoarthritis genetics
Osteoarthritis metabolism
Osteoarthritis pathology
Proteoglycans metabolism
RNA Interference
RNA, Small Interfering genetics
Synovial Membrane cytology
Vascular Endothelial Growth Factor A genetics
Vascular Endothelial Growth Factor A metabolism
Arthritis genetics
Gene Expression
Neoplasm Proteins genetics
Proteoglycans genetics
Synovial Membrane metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 11
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 25483913
- Full Text :
- https://doi.org/10.3892/mmr.2014.3057