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A novel method to detect Listeria monocytogenes via superparamagnetic lateral flow immunoassay.
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2015 Jan; Vol. 407 (2), pp. 529-35. Date of Electronic Publication: 2014 Dec 09. - Publication Year :
- 2015
-
Abstract
- A novel strip test system combining immunomagnetic separation with lateral flow immunoassay (LFIA) was established for the accurate detection of Listeria monocytogenes. In this system, a pair of matched monoclonal antibodies was used to construct a sandwich immunoassay, in which superparamagnetic particles were coupled with one of the antibodies as a labeled antibody to capture the target bacteria, while the other antibody was immobilized on the detection zone. After a 20-min reaction, the strips were analyzed by a novel instrument which could detect the magnetic signal of the immunocomplex in a magnetic field. Sensitivity evaluation showed that the limit of detection (LOD) of the superparamagnetic LFIA system for L. monocytogenes was 10(4) CFU/mL, which was at least one log lower than conventional LFIA. No cross-reaction was observed when Salmonella, Escherichia coli O157:H7, or three types of harmless Listeria strains were tested. Further evaluation with actual food samples indicated that the superparamagnetic LFIA system showed 100 % concordance with real-time PCR. Therefore, this novel superparamagnetic LFIA system could be used as a rapid, sensitive, and specific method for the detection of L. monocytogenes.
- Subjects :
- Antibodies, Immobilized chemistry
Antibodies, Monoclonal
Cross Reactions
Equipment Design
Escherichia coli O157 immunology
Food Contamination analysis
Immunoassay instrumentation
Immunomagnetic Separation methods
Limit of Detection
Real-Time Polymerase Chain Reaction
Salmonella immunology
Sensitivity and Specificity
Food Microbiology methods
Immunoassay methods
Listeria monocytogenes genetics
Listeria monocytogenes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 407
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25486917
- Full Text :
- https://doi.org/10.1007/s00216-014-8276-8