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Cloning, expression and purification of outer membrane protein (OmpA) of Burkholderia pseudomallei and evaluation of its potential for serodiagnosis of melioidosis.
- Source :
-
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2015 Feb; Vol. 81 (2), pp. 79-84. Date of Electronic Publication: 2014 Oct 22. - Publication Year :
- 2015
-
Abstract
- Melioidosis is an emerging infectious disease in India and caused by gram-negative, soil saprophyte bacteria Burkholderia pseudomallei. This disease is endemic in Southeast Asia and northern Australia, and sporadic cases of melioidosis are also reported from southern states of India. The present study reports the cloning, expression, and purification of recombinant protein outer membrane protein A (OmpA) of B. pseudomallei and its evaluation in indirect enzyme-linked immunosorbent assay (ELISA) format with 87 serum samples collected from Manipal, Karnataka, India. Twenty-three samples from culture confirmed cases (n=23) of melioidosis, 25 serum samples from patients of other febrile illness and pyrexia of unknown origin (n=25), and 39 serum samples from healthy blood donors (n=39) from Kasturba Medical College, Manipal, were tested in this assay format. The assay showed sensitivity of 82.6% and specificity of 93.75%. The recombinant OmpA based indirect ELISA will be a useful tool for serodiagnosis of melioidosis in large scale rapid screening of clinical samples.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Cloning, Molecular
Enzyme-Linked Immunosorbent Assay methods
Gene Expression
Humans
Recombinant Proteins genetics
Recombinant Proteins immunology
Recombinant Proteins isolation & purification
Sensitivity and Specificity
Serologic Tests methods
Antibodies, Bacterial blood
Antigens, Bacterial genetics
Antigens, Bacterial immunology
Antigens, Bacterial isolation & purification
Bacterial Outer Membrane Proteins genetics
Bacterial Outer Membrane Proteins immunology
Bacterial Outer Membrane Proteins isolation & purification
Burkholderia pseudomallei immunology
Melioidosis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0070
- Volume :
- 81
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Diagnostic microbiology and infectious disease
- Publication Type :
- Academic Journal
- Accession number :
- 25488273
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2014.10.003