Back to Search
Start Over
miRNA-34a underexpressed in Merkel cell polyomavirus-negative Merkel cell carcinoma.
miRNA-34a underexpressed in Merkel cell polyomavirus-negative Merkel cell carcinoma.
- Source :
-
Virchows Archiv : an international journal of pathology [Virchows Arch] 2015 Mar; Vol. 466 (3), pp. 289-95. Date of Electronic Publication: 2014 Dec 10. - Publication Year :
- 2015
-
Abstract
- Merkel cell polyomavirus (MCV) is frequently detectable in Merkel cell carcinoma (MCC) tumors, but the significance of MCV infection is not yet totally understood. Thus far, no key regulatory miRNA has been identified for MCC tumorigenesis. However, distinct miRNA expression profiles have been suggested for MCV-positive and MCV-negative tumors. We used microarray hybridization to identify miRNA expression differences in MCC tumor samples according to MCV status and further validated these results by quantitative reverse transcription polymerase chain reaction (qRT-PCR). When compared with MCV-negative tumors, we detected overexpression of miR-34a, miR-30a, miR-142-3p, and miR-1539 in those MCV positives. In addition, slight underexpression was detectable in MCV-positive tumors of miR-181d. We confirmed the distinct expression of miRNAs in MCV-positive and MCV-negative tumors and confirmed statistically significant underexpression of miR-34a in MCV-negative tumors by both array analysis and qRT-PCR. Neither tumor location nor development of metastases affected miRNA expression.
- Subjects :
- Aged
Aged, 80 and over
Biomarkers, Tumor genetics
Carcinoma, Merkel Cell pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Male
MicroRNAs genetics
Microarray Analysis
Middle Aged
Reproducibility of Results
Retrospective Studies
Skin Neoplasms pathology
Biomarkers, Tumor metabolism
Carcinoma, Merkel Cell metabolism
Down-Regulation
Merkel cell polyomavirus
MicroRNAs metabolism
Skin Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-2307
- Volume :
- 466
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Virchows Archiv : an international journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 25491743
- Full Text :
- https://doi.org/10.1007/s00428-014-1700-9