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Squaraine-based polymer dots with narrow, bright near-infrared fluorescence for biological applications.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2015 Jan 14; Vol. 137 (1), pp. 173-8. Date of Electronic Publication: 2014 Dec 26. - Publication Year :
- 2015
-
Abstract
- This article describes the design and development of squaraine-based semiconducting polymer dots (Pdots) that show large Stokes shifts and narrow-band emissions in the near-infrared (NIR) region. Fluorescent copolymers containing fluorene and squaraine units were synthesized and used as precursors for preparing the Pdots, where exciton diffusion and likely through-bond energy transfer led to highly bright and narrow-band NIR emissions. The resulting Pdots exhibit the emission full width at half-maximum of ∼36 nm, which is ∼2 times narrower than those of inorganic quantum dots in the same wavelength region (∼66 nm for Qdot705). The squaraine-based Pdots show a high fluorescence quantum yield (QY) of 0.30 and a large Stokes shift of ∼340 nm. Single-particle analysis indicates that the average per-particle brightness of the Pdots is ∼6 times higher than that of Qdot705. We demonstrate bioconjugation of the squaraine Pdots and employ the Pdot bioconjugates in flow cytometry and cellular imaging applications. Our results suggest that the narrow bandwidth, high QY, and large Stokes shift are promising for multiplexed biological detections.
- Subjects :
- Cyclobutanes chemical synthesis
Flow Cytometry
Humans
Infrared Rays
MCF-7 Cells
Molecular Structure
Particle Size
Phenols chemical synthesis
Polymers chemical synthesis
Semiconductors
Surface Properties
Cyclobutanes chemistry
Fluorescence
Neoplasms pathology
Phenols chemistry
Polymers chemistry
Quantum Dots
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5126
- Volume :
- 137
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 25494172
- Full Text :
- https://doi.org/10.1021/ja5123045