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Improving xylitol production through recombinant expression of xylose reductase in the white-rot fungus Phanerochaete sordida YK-624.

Authors :
Hirabayashi S
Wang J
Kawagishi H
Hirai H
Source :
Journal of bioscience and bioengineering [J Biosci Bioeng] 2015 Jul; Vol. 120 (1), pp. 6-8. Date of Electronic Publication: 2014 Dec 26.
Publication Year :
2015

Abstract

We generated an expression construct consisting of the xylose reductase (XR) gene (xr) from Phanerochaete chrysosporium. Transformant X7 exhibited increased xylitol production and markedly higher XR activities than the wild-type strain. RT-PCR analysis demonstrated that the increased XR activity was associated with constant expression of the recombinant xr gene.<br /> (Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1347-4421
Volume :
120
Issue :
1
Database :
MEDLINE
Journal :
Journal of bioscience and bioengineering
Publication Type :
Academic Journal
Accession number :
25547244
Full Text :
https://doi.org/10.1016/j.jbiosc.2014.11.024