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α-Naphthoflavone modulates inflammatory response in adipocytes-macrophages interaction through NFκB signaling.

α-Naphthoflavone modulates inflammatory response in adipocytes-macrophages interaction through NFκB signaling.

Authors :
Sun Y
Xie M
Huang T
Zhang X
Lei S
Shi Q
Wang S
Fan C
Zhang J
Source :
International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2014 Oct 15; Vol. 7 (11), pp. 7768-74. Date of Electronic Publication: 2014 Oct 15 (Print Publication: 2014).
Publication Year :
2014

Abstract

Objective: Our previous study demonstrated that α-naphthoflavone (α-NF) inhibits mouse 3T3-L1 pre-adipocytes differentiation via PPARγ, a key transcription factor in adipogenesis. Due to the critical role of inflammation in adipogenesis, we speculated that the suppression role of α-NF in adipogenesis might involve in modulation of cytokines secretion raised by adipocyte differentiation cocktail. Therefore, the present study aims to investigate the role of α-NF in modulating of inflammatory response during adipocytes differentiation and adipocyte-macrophage interaction.<br />Methods: Conditioned medium from different doses of α-NF treated 10-day differentiated 3T3-L1 adipocytes were collected to culture RAW264.7 macrophages. Conditioned medium from activated macrophages and α-NF pre-treated macrophage were used to investigate the effects of α-NF in adipocytes differentiation. Cultured cells and medium were harvested for RT-PCR, Western blot and ELISA.<br />Results: α-NF dose-dependently decreased TNF-α and IL-6 and increased IL-10 expression induced by IDM (Insulin, dexamethasone, isobutylmethylxanthine) in 3T3-L1 pre-adipocytes. Conditioned medium from α-NF treated 3T3-L1 differentiated cells inhibited inflammatory response in mouse macrophage cell line RAW264.7 in contrast to IDM control medium. NFĸB activation elicited by IDM was suppressed by α-NF in a dose-response manner. Consequently, decreased TNF-α and increased IL-10 secretion, downstream targets of NFĸB signaling pathway, were observed with α-NF in macrophages. Finally, Conditioned medium from α-NF pre-treated, LPS-activated macrophages ameliorated the suppression of 3T3-L1 adipogenesis by LPS activated macrophages.<br />Conclusion: Our results suggest that α-NF regulates inflammation response in both adipocytes and macrophages and adipocyte-macrophage interaction which contributes to pre-adipocyte differentiation.

Details

Language :
English
ISSN :
1936-2625
Volume :
7
Issue :
11
Database :
MEDLINE
Journal :
International journal of clinical and experimental pathology
Publication Type :
Academic Journal
Accession number :
25550814