Back to Search
Start Over
Dexamethasone disrupts cytoskeleton organization and migration of T47D Human breast cancer cells by modulating the AKT/mTOR/RhoA pathway.
- Source :
-
Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2014; Vol. 15 (23), pp. 10245-50. - Publication Year :
- 2014
-
Abstract
- Background: Glucocorticoids are commonly co-administered with chemotherapy to prevent drug-induced allergic reactions, nausea, and vomiting, and have anti-tumor functions clinically; however, the distinct effects of GC on subtypes of tumor cells, especially in breast cancer cells, are still not well understood. In this study, we aimed to clarify the effect of GC on subtypes of T47D breast cancer cells by focusing on apoptosis, cell organization and migration, and underluing molecular mechanisms.<br />Materials and Methods: The cell scratch test was performed to observe the cell migration rate in T47D cells treated with dexamethasone (Dex). Hoechst and MTT assays were conducted to detect cell survival and rhodamine-labeled phalloidin staining to observe cytoskeleton dynamics. Related factors in the AKT/mTOR pathway were determined by Western blotting.<br />Results: Dex treatment could effectively inhibit T47D breast cancer cell migration with disruption of the cytoskeletal dynamic organization. Moreover, the effect of Dex on cell migration and cytoskeleton may be mediated by AKT/ mTOR/RhoA pathway. Although Dex inhibited T47D cell migration, it alone may not induce cell apoptosis in T47D cells.<br />Conclusions: Dex in T47D human breast cancer cells could effectively inhibit cell migration by disrupting the cytoskeletal dynamic organization, which may be mediated by the AKT/mTOR/RhoA pathway. Our work suggests that glucocorticoid/Dex clinical use may prove helpful for the treatment of breast cancer metastasis.
- Subjects :
- Cell Line, Tumor
Cell Survival drug effects
Female
Humans
Proto-Oncogene Proteins c-akt metabolism
Signal Transduction drug effects
TOR Serine-Threonine Kinases metabolism
rhoA GTP-Binding Protein metabolism
Antineoplastic Agents, Hormonal pharmacology
Breast Neoplasms metabolism
Cell Movement drug effects
Cytoskeleton drug effects
Dexamethasone pharmacology
Proto-Oncogene Proteins c-akt drug effects
TOR Serine-Threonine Kinases drug effects
rhoA GTP-Binding Protein drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2476-762X
- Volume :
- 15
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Asian Pacific journal of cancer prevention : APJCP
- Publication Type :
- Academic Journal
- Accession number :
- 25556455
- Full Text :
- https://doi.org/10.7314/apjcp.2014.15.23.10245