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Galactose targeted pH-responsive copolymer conjugated with near infrared fluorescence probe for imaging of intelligent drug delivery.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2015 Jan 28; Vol. 7 (3), pp. 2104-15. Date of Electronic Publication: 2015 Jan 16. - Publication Year :
- 2015
-
Abstract
- Theranostic polymeric nanomaterials are of special important in cancer treatment. Here, novel galactose targeted pH-responsive amphiphilic multiblock copolymer conjugated with both drug and near-infrared fluorescence (NIR) probe has been designed and prepared by a four-steps process: (1) ring-opening polymerization (ROP) of N-carboxy anhydride (NCA) monomers using propargylamine as initiator; (2) reversible addition-fragmentation chain transfer (RAFT) polymerization of oligo(ethylene glycol) methacrylate (OEGMA) and gal monomer by an azido modified RAFT agent; (3) combing the obtained two polymeric segments by click reaction; (4) NIR copolymer prodrug was synthesized by chemical linkage of both cyanine dye and anticancer drug doxorubicin to the block copolymer via amide bond and hydrazone, respectively. The obtained NIRF copolymers were characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), and its was measured by means of micelles dynamic light scattering (DLS), field emission transmission electron microscopy (FETEM), and UV-vis and fluorescence spectrophotometry. The prodrug has strong fluorescence in the near-infrared region, and a pH sensitive drug release was confirmed at pH of 5.4 via an in vitro drug release experiment. Confocal laser scanning microscopy (CLSM) and flow cytometry experiments of the prodrug on both HepG2 and NIH3T3 cells reveal that the galactose targeted polymeric prodrug shows a fast and enhanced endocytosis due to the specific interaction for HepG2 cells, indicating the as-prepared polymer is a candidate for theranosis of liver cancer.
- Subjects :
- Animals
Carbon Dioxide chemistry
Doxorubicin pharmacology
Drug Delivery Systems methods
Fluorescence
Hep G2 Cells
Humans
Mice
Molecular Imaging
Molecular Probes chemical synthesis
NIH 3T3 Cells
Polymerization
Polymers chemical synthesis
Doxorubicin chemistry
Drug Carriers chemistry
Drug Delivery Systems instrumentation
Galactose chemistry
Molecular Probes chemistry
Polymers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 7
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 25569169
- Full Text :
- https://doi.org/10.1021/am508291k