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Adhesion and growth of human bone marrow mesenchymal stem cells on precise-geometry 3D organic-inorganic composite scaffolds for bone repair.
- Source :
-
Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2015 Mar; Vol. 48, pp. 301-9. Date of Electronic Publication: 2014 Dec 05. - Publication Year :
- 2015
-
Abstract
- Engineering biomaterial scaffolds that promote attachment and growth of mesenchymal stem cells in three dimensions is a crucial parameter for successful bone tissue engineering. Towards this direction, a lot of research effort has focused recently into the development of three-dimensional porous scaffolds, aiming to elicit positive cellular behavior. However, the fabrication of three-dimensional tissue scaffolds with a precise geometry and complex micro- and nano-features, supporting cell in-growth remains a challenge. In this study we report on a positive cellular response of human bone marrow-derived (BM) mesenchymal stem cells (MSCs) onto hybrid material scaffolds consisting of methacryloxypropyl trimethoxysilane, zirconium propoxide, and 2-(dimethylamino)ethyl methacrylate (DMAEMA). First, we use Direct fs Laser Writing, a 3D scaffolding technology to fabricate the complex structures. Subsequently, we investigate the morphology, viability and proliferation of BM-MSCs onto the hybrid scaffolds and examine the cellular response from different donors. Finally, we explore the effect of the materials' chemical composition on cell proliferation, employing three different material surfaces: (i) a hybrid consisting of methacryloxypropyl trimethoxysilane, zirconium propoxide and 50mol% DMAEMA, (ii) a hybrid material comprising methacryloxypropyl trimethoxysilane and zirconium propoxide, and (iii) a purely organic polyDMAEMA. Our results show a strong adhesion of BM-MSCs onto the hybrid material containing 50% DMAEMA from the first 2h after seeding, and up to several days, and a proliferation increase after 14 and 21days, similar to the polystyrene control, independent of cell donor. These findings support the potential use of our proposed cell-material combination in bone tissue engineering.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- 1-Propanol
Bone and Bones
Cell Culture Techniques instrumentation
Cell Culture Techniques methods
Cell Proliferation
Guided Tissue Regeneration
Humans
Mesenchymal Stem Cells immunology
Methacrylates
Microscopy, Confocal methods
Microscopy, Electron, Scanning
Silanes
Zirconium
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells physiology
Tissue Engineering methods
Tissue Scaffolds
Subjects
Details
- Language :
- English
- ISSN :
- 1873-0191
- Volume :
- 48
- Database :
- MEDLINE
- Journal :
- Materials science & engineering. C, Materials for biological applications
- Publication Type :
- Academic Journal
- Accession number :
- 25579927
- Full Text :
- https://doi.org/10.1016/j.msec.2014.12.007