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S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2015 Mar; Vol. 35 (3), pp. 675-83. Date of Electronic Publication: 2015 Jan 12. - Publication Year :
- 2015
-
Abstract
- Colorectal cancer (CRC) is a genetically heterogeneous disease with distinct morphological patterns. It has been shown that polypoid and ulcerative CRC displays different genetic alterations. In the present study, we aimed to investigate genes with differential expression patterns between ulcerative and polypoid CRC. cDNA microarray analysis was performed to compare the gene expression profiles in samples of ulcerative and polypoid CRC with paired normal mucosa samples. Potential candidate genes were further validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot analysis and immunohistochemistry. The epigenetic regulation of gene expression was investigated using methylation-specific PCR (MSP). cDNA microarray analysis identified 11 upregulated and 14 downregulated genes which were differentially expressed in samples from both tumor types compared to the matched normal mucosa samples. Among these, S100P was the only upregulated gene preferentially associated with polypoid CRC (P=0.032). The samples of polypoid CRC displayed significantly higher S100P protein and mRNA expression levels than the samples of ulcerative CRC (P<0.05, respectively). Using semi-quantitative immunohistochemical analyses, S100P overexpression was found to be preferentially associated with polypoid CRC (24/30 vs. 14/40, P<0.001). The relative methylation level determined by MSP did not differ significantly between the samples of polypoid and ulcerative CRC (43.36 vs. 49.10%, P=0.168), indicating that promoter hypomethylation was not directly related to the upregulation of S100P mRNA. Our results demonstrate that the upregulation of S100P mRNA and protein expression is a predominant characteristic in polypoid CRC, whereas ulcerative CRC presents with a wide range of expression levels, indicating that S100P overexpression is not a key determinant in conferring invasion properties. The clinicopathological significance of S100P in CRC requires further investigation in well-controlled studies.
- Subjects :
- Adult
Aged
Calcium-Binding Proteins metabolism
DNA Methylation
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Intestinal Mucosa metabolism
Intestinal Mucosa pathology
Male
Middle Aged
Neoplasm Grading
Neoplasm Proteins metabolism
Neoplasm Staging
Promoter Regions, Genetic
Transcriptome
Tumor Burden
Calcium-Binding Proteins genetics
Colorectal Neoplasms genetics
Colorectal Neoplasms pathology
Neoplasm Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1791-244X
- Volume :
- 35
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 25585623
- Full Text :
- https://doi.org/10.3892/ijmm.2015.2065