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Overexpression of interleukin-18 protein reduces viability and induces apoptosis of tongue squamous cell carcinoma cells by activation of glycogen synthase kinase-3β signaling.
- Source :
-
Oncology reports [Oncol Rep] 2015 Mar; Vol. 33 (3), pp. 1049-56. Date of Electronic Publication: 2015 Jan 15. - Publication Year :
- 2015
-
Abstract
- The aim of this study was to investigate the effects of interleukin-18 (IL-18) expression on regulating the viability and apoptosis of tongue squamous cell carcinoma (TSCC) cells in vitro and examine the underlying molecular events. Human IL-18 cDNA was cloned into the vector pcDNA3.1 (+) and transfected into CRL-1623™ cells. Quantitative reverse transcription-PCR (RT-qPCR), western blot analysis, immunofluorescence, cell viability MTT assay, flow cytometric Annexin V/propidium iodide (PI), Giemsa staining, and caspase-3 activity assay were performed. The data showed that overexpression of IL-18 protein reduced TSCC cell viability by inducing apoptosis. Compared with cells transfected with the control vector, IL-18 expression activated caspase-3, -7, and -9 by inducing their cleavage and increased the expression of interferon (IFN)-γ and cytochrome c mRNA, but reduced cyclin D1 and A1 expression in TSCC cells. IL-18 expression upregulated the expression and phosphorylation of glycogen synthase kinase (GSK)-3β protein in CRL1623 cells, whereas the selective GSK-3β inhibitor kenpaullone antagonized the effects of IL-18 protein on TSCC cells in vitro. The results indicated that IL-18 played an important role in the inhibition of TSCC cell growth and may be further investigated as a novel therapeutic target against TSCC.
- Subjects :
- Blotting, Western
Carcinoma, Squamous Cell metabolism
Cell Survival physiology
Enzyme Activation physiology
Flow Cytometry
Fluorescent Antibody Technique
Glycogen Synthase Kinase 3 beta
Humans
Real-Time Polymerase Chain Reaction
Tongue Neoplasms metabolism
Transfection
Apoptosis physiology
Carcinoma, Squamous Cell pathology
Glycogen Synthase Kinase 3 metabolism
Interleukin-18 biosynthesis
Signal Transduction physiology
Tongue Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 33
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 25591548
- Full Text :
- https://doi.org/10.3892/or.2015.3724